| Solution Information | help | |
| Enzyme: | Large T antigen | |
| inhibitor: | BDBM58982 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Protocol: Cytotoxicity was evaluated using Madin Darby Canine Kidney Cells (MDCK, ATCC CCL-34). This assay was conducted with 999 of the 1205 compounds designated as active in the confirmation and counterscreens. Compounds were plated in dose response format (in less than 1% DMSO final concentration), followed by the addition of cells. Cells were grown and incubated with compounds in DMEM/pen/strep/L-glutamine/10% FBS, for 72 hr at 37 degrees C/5% CO2. At the end of the incubation time cells were assayed for viability using Cell Titer GLO (Promega). Compounds that were considered cytotoxic resulted in a decreased luminescent signal. Compounds that interfere with luciferase will appear as toxic in this screen. | |
| If you find an error in this entry please send us an E-mail | ||